2231-3915 (Online)
2231-3907 (Print)

Author(s): Nimmy Abraham, Chitra Namachivayam, Sangeetha Sundaramoorthy


DOI: 10.52711/2231-3915.2021.00010   

Address: Nimmy Abraham, Chitra Namachivayam, Sangeetha Sundaramoorthy
Department of Microbiology, St. Peters Institute of Higher Education and Research Avadi, Chennai-600054, India.
*Corresponding Author

Published In:   Volume - 11,      Issue - 2,     Year - 2021

The gut microbiome is not a silent ecosystem but exerts several physiological and immunological functions. For many decades, lactobacilli have been used as an effective therapy for treatment of several pathological conditions displaying an overall positive safety profile. The present article gives an account of updated information on pharmacological properties such as anti-fungal activity, anti-viral activity, Activity on vaginal pathogens, immunomodulatory activity, Cholesterol lowering activity and various other important properties. Because of blocking effect, selected probiotic lactobacilli may be used as biological preservative, so, the aim of this study was to present some data on lactobacillus as probiotic bacteria. Lactobacilli originally isolated from meat products are the best candidates as probiotic bacteria to improve the microbiological safety of these foods. Finally, we need to determine the adequate number of bacteria to be delivered in order to achieve the best clinical efficacy decreasing the risk of side effects.

Cite this article:
Nimmy Abraham, Chitra Namachivayam, Sangeetha Sundaramoorthy. Lactobacillus- An friendly Bacteria. International Journal of Technology. 2021; 11(2):70-7. doi: 10.52711/2231-3915.2021.00010

Nimmy Abraham, Chitra Namachivayam, Sangeetha Sundaramoorthy. Lactobacillus- An friendly Bacteria. International Journal of Technology. 2021; 11(2):70-7. doi: 10.52711/2231-3915.2021.00010   Available on:

1.    Abdel-Daim, M. Ashor, N. Hassouna, M. Hafez, and M. Aboulwafa, “Screening of Lactobacillus isolates for their probiotic potential, ” Archives of Clinical Microbiology, vol. 3, no. 5, 2012.16
2.    Andreu, A. E. Stapleton, C. L. Fennell, S. L. Hillier, and W. E. Stamm, “Hemagglutination, adherence, and surface properties of vaginal Lactobacillus species, ” Journal of Infectious Diseases, vol. 171, no. 5, pp. 1237–1243, 1995.
3.    Giorgi, S. Torriani, F. Dellaglio, G. Bo, E. Stola, and L. Bernuzzi, “Identification of vaginal lactobacilli from asymptomatic women, ” Microbiologica, vol. 10, no. 4, pp. 377–384, 1987.
4.    L. Servin, “Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens, ” FEMS Microbiology Reviews, vol. 28, no. 4, pp. 405–440, 2004.
5.    Ahmadi E, Alizadeh-Navaei R, Rezai MS. Efficacy of probiotic use in acute rotavirus diarrhea in children: A systematic review and meta-analysis. Caspian journal of internal medicine 6 (2015): 187.
6.    Amira Abdel-Daim, Nadia Hassouna, Mohamed Hafez, Mohamed Seif Aldeen Ashor,  and Mohammad M. Aboulwafa. Antagonistic Activity of Lactobacillus Isolates against Salmonella typhi In Vitro.
7.    Anandaraj, B., Thivakaran, P. 2010. Isolation and production of biosurfactant producing organism from oil spilled soil. J. Biosci. Technol., 1(3): 120 126.
8.    Anayata Sharma, Jitesh Soni, Gurveer Kaur, Jaspreet Kaur. A Study on biosurfactant production in Lactobacillus and Bacillus sp. ISSN: 2319-7706 Volume 3 Number 11 (2014) pp. 723-733
9.    Begley M, Gahan CGM, hill C. The interaction between bacteria and bile. FEMS Microbiol Rev 2005; 29: 625-651.
10.    Belviso et al., 2009Belviso, S., Giordano, M., Dolci, P., and Zeppa, G. In vitro cholesterol-lowering activity of Lactobacillus plantarum and Lactobacillus paracasei strains isolated from the Italian Castelmagno PDO cheese. Dairy Sci. Technol. 2009; 89: 169– 176
11.    BhuvanSaud , Pratima Pandey, Govinda Paudel, Gunaraj Dhungana, Vikram Shrestha. In-Vitro Antibacterial Activity of Probiotic Against Human Multidrug Resistant Pathogens Arch Vet Sci Med 2020; 3 (1): 31-39 DOI: 10.26502/avsm.013
12.    Albano S. Morandi, T. Silvetti, M.C. Casiraghi, F. Manini M. Brasca, Lactic acid bacteria with cholesterol-lowering properties for dairy applications: In vitro and in situ activity.
13.    M. Parry, T. T. Hein, G. Dougan, N. J. White, and J. J. Farrar, “Typhoid fever, ” The New England Journal of Medicine, vol. 347, no. 22, pp. 1770–1782, 2002.
14.    Cadieux PA, Burton J, Devillard E, et al. (2009) Lactobacillus by-products inhibit the growth and virulence of uropathogenic Escherichia coli. Journal of Physiology and Pharmacology 60:13–18.
15.    Chantharasophon, K., T. Warong, P. Mapatsa and V. Leelavatcharamas, 2011. High Potential Probiotic Bacillus species from gastrointestinal Tract of Nile Tilapia (Oreochromis niloticus). Biotechnology, 10: 489-505
16.    Collins M, Glenn D, Gibson R (1999). Probiotics, prebiotics and symbiotics: Approches for modulating the microbial ecology of the gut. American J Clin Nutri, 69(5): 1052s-57s.
17.    I. Henry, “Broth microdilution MIC test, ” in Clinical Microbiology Procedure Handbook, D. I. Henry, Ed., vol. 2, ASM Press, Washington, DC, USA, 2007. 23
18.    J. Okkers, L. M. T. Dicks, M. Silvester, J. J. Joubert, and H. J. Odendaal, “Characterization of pentocin TV35b, a bacteriocin-like peptide isolated from Lactobacillus pentosus with a fungistatic effect on Candida albicans, ” Journal of Applied Microbiology, vol. 87, no. 5, pp. 726–734, 1999.
19.    De Vrese M, Schrezenmeir J. Probiotics, prebiotics, and synbiotics. Adv BiochemEngBiotechnol 2008; 111(8): 1.
20.    Duraiswamy, H., S. Nallaiyan, J. Nelson, P.R. Samy, M. Johnson and I. Varaprasadam, 2010. The effect of extracts of Selaginella involvens and Selaginella inaequalifolia leaves on poultry pathogens. Asian Pac. J. Trop. Med., 3: 678-681.
21.    Eade CR, Diaz C, Wood MP, et al. (2012) Identification and characterization of bacterial vaginosis-associated pathogens using a comprehensive cervical-vaginal epithelial coculture assay. PLoS One 7: e50106.
22.    Elamathy, S. and D. Kanchana, 2012. Antimicrobial activity of lactic acid bacteria isolated from goat meat against various pathogenic bacteria. Int. J. Pharmaceut. Biol. Arch., 3: 930-932.
23.    Valerio, P. Lavermicocca, M. Pascale, and A. Visconti, “Production of phenyllactic acid by lactic acid bacteria: an approach to the selection of strains contributing to food quality and preservation, ” FEMS Microbiology Letters, vol. 233, no. 2, pp. 289–295, 2004.
24.    FAO/WHO, “Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria: report of a Joint FAO/WHO expert consultation on evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria, ” Tech. Rep., Food and Agriculture Organization/World Health Organization, Cordoba, Argentina, 2001. 1
25.    FatenKachouri, Hamida Ksontini, Manel Kraiem, KhaoulaSetti, Manel Mechmeche, and Moktar Hamdi.Involvement of antioxidant activity of Lactobacillus plantarum on functional properties of olive phenolic compounds.
26.    Food and Agriculture Organization and World Health Organization Expert Consultation. Evaluation of health and nutritional properties of powder milk and live lactic acid bacteria. Córdoba, Argentina: Food and Agriculture Organization of the United Nations and World Health Organization; 2001.
27.    W. Tannock, “Analysis of the intestinal microflora: a renaissance, ” Antonie van Leeuwenhoek, vol. 76, no. 1–4, pp. 265–278, 1999.
28.    Galdeano C, Perdigon G. Role of viability of probiotic strains in their persistence in the gut and in mucosal immune stimulation. J Appl Microbiol 2004; 97(4): 673.
29.    Hickson M. Probiotics in the prevention of antibiotic-associated diarrhoea and Clostridium difficile infection. Therapeutic advances in gastroenterology 4 (2011): 185-197.
30.    M. Helander, A. von Wright, and T.-M. Mattila-Sandholm, “Potential of lactic acid bacteria and novel antimicrobials against gramnegative bacteria, ” Trends in Food Science and Technology, vol. 8, no. 5, pp. 146–150, 1997.
31.    Iqbal MZ, Qadir MI, Hussain T, Janbaz KH, Khan YH, Ahmad B. Review: probiotics and their beneficial effects against various diseases. Pak J Pharm Sci 2014; 27: 405-415. 6
32.    Irfan A. Rather, Kwang-Ho Choi, Vivek K. Bajpai and Yong-Ha Park.Antiviral mode of action of Lactobacillus plantarum YML009 on Influenza virus H1N1.Bangladesh Journal of Pharmacology 10(2):475 · March 2015.
33.    D. Legan, “Mould spoilage of bread: the problem and some solutions, ” International Biodeterioration and Biodegradation, vol. 32, no. 1–3, pp. 33–53, 1993.
34.    Magnusson, K. Ström, S. Roos, J. Sjögren, and J. Schnürer, “Broad and complex antifungal activity among environmental isolates of lactic acid bacteria, ” FEMS Microbiology Letters, vol. 219, no. 1, pp. 129–135, 2003.
35.    K.J. Ayantola and M.K. Oladunmoye. Antibacterial Activity of Lactobacillus Species Isolated from Poultry Waste (Droppings) Against Poultry Pathogens . DOI: 10.3923/crpsaj.2016.7.12
36.    Kaenhammer TR (2000). Probiotic bacteria: today and tomorrow. J Nut, 130(2S suppl): 415S-16S. 2.
37.    Kao TH, Chen BHJ .Functional components in soybean cake and their effects on antioxidant activity. Agric Food Chem. 2006 Oct 4; 54(20):7544-55.
38.    Kawashima T, Hayashi K, Kosaka A, Kawashima M, Igarashi T, Tsutsui H, et al. Lactobacillus plantarum strain YU from fermented foods activates Th1 and protective immune responses. Int Immunopharmacol 2011; 11(12): 2017–24.
39.    Kechagia M, Basoulis D, Konstantopoulou S, et al. Health benefits of probiotics: a review. ISRN nutrition (2013).
40.    Kikuchi Y, Kunitoh-Asari A, Hayakawa K, Imai S, Kasuya K, Abe K, Adachi Y, Fukudome S, Takahashi Y, Hachimura S. 2014. Oral administration of Lactobacillus plantarum strain AYA enhances IgA secretion and provides survival protection against in-fluenza virus infection in mice. PLoS One 9, e86416.
41.    Kimoto et al., 2002 Kimoto, H., Ohmomo, S., and Okamoto, T. Cholesterol removal from media by lactococci. J. Dairy Sci. 2002; 85: 3182–3188 (12512591)
42.    Klaver and Van Der Meer, 1993 Klaver, F.A. and Van Der Meer, R. The assumed assimilation of cholesterol by Lactobacilli and Bifidobacterium bifidum is due to their bile salt-deconjugating activity. Appl. Environ. Microbiol. 1993; 59: 1120–1124 (8489229)
43.    Kumar, R., S. Pandey, P. Kapoor, S. Awasthi and T. Bhatnagar, 2014. Isolation and characterization of endemic strains of Lactobacillus sp. and evaluation of their probiotic activity. Int. J. Curr. Microbiol. Applied Sci, 3: 907-916.
44.    Srivastava and P. Aggarwal, “Multidrug resistant Salmonella typhi in Delhi, ” Indian Journal of Medical Microbiology, vol. 12, pp. 102–105, 1994.
45.    Lilly DM, Stillwell RH Probiotics: growthpromoting factors produced by microorganisms. Science 147 (1965): 747-748.
46.    Lucia Bertuccini, Rosario Russo, Francesca Iosi and Fabiana. Effects of Lactobacillus rhamnosus and Lactobacillus acidophilus on bacterial vaginal pathogens Superti1 International Journal of Immunopathology and Pharmacology 2017, Vol. 30(2) 163–167
47.    V. Arasu, D. H. Kim, P. I. Kim et al., “In vitro antifungal, probiotic and antioxidant properties of novel Lactobacillus plantarum K46 isolated from fermented sesame leaf, ” Annals of Microbiology, vol. 64, no. 3, pp. 1333–1346, 2013.
48.    Ma B, Forney LJ and Ravel J (2012) Vaginal microbiome: Rethinking health and disease. Annual Review in Microbiology 66: 371– 389
49.    Manzoor A, Ul-Haq I, Baig S, et al. Efficacy of locally isolated lactic acid bacteria against antibiotic-resistant uropathogens. Jundishapur journal of microbiology 9 (2016): 18952.
50.    Mastromarino P, Vitali B and Mosca L (2013) Bacterial vaginosis: A review on clinical trials with probiotics. New Microbiologica 36: 229–238.
51.    Moayyedi P, Ford AC, Talley NJ, et al. The efficacy of probiotics in the treatment of irritable bowel syndrome: a systematic review. Gut 59 (2010): 325-332.
52.    Pan XD, Chen FQ, Wu TX. The acid, bile tolerance and antimicrobial property of Lactobacillus acidophilus NIT. Food Control 2009; 20: 598-602.
53.    Paturi G, Phillips M, Jones M, and Kailasapathy K. Immune enhancing effects of Lactobacillus acidophilus LAFTI L10 and Lactobacillus paracasei LAFTI L26 in mice. Int J Food Microbiol 2007; 115(1): 115–118.
54.    Pereira and Gibson, 2002Pereira, D.I.A. and Gibson, G.R. Cholesterol assimilation by lactic acid bacteria and bifidobacteria isolated from the human gut. Appl. Environ. Microbiol. 2002; 68: 4689–4693 (12200334)
55.    Prabhurajeshwar C, Chandrakanth RK. Probiotic potential of Lactobacilli with antagonistic activity against pathogenic strains: An in vitro validation for the production of inhibitory substances. biomedical journal 40 (2017): 270-283.
56.    Rafter J, Bennett M, Caderni G, et al. Dietary synbiotics reduce cancer risk factors in polypectomized and colon cancer patients. The American journal of clinical nutrition 85 (2007): 488-496.
57.    Reuter G (2001). Probiotics-Possibilities and limitations of their application in food, animal feed, and in pharmaceutical preparations for men and animals. Berl Munch TierarztlWochenschr, 114(11-12): 410-9.
58.    Rigaux P, Daniel C, Hisbergues M, Muraille E, Hols P, Pot B, et al. Immunomodulatory properties of Lactobacillus plantarum, and its use as a recombinant vaccine against mite allergy. Allergy 2009; 64(3): 406–14.
59.    Rizzo A, Losacco A, Carratelli CR, Domenico MD, Bevilac-qua, N. Lactobacillus plantarum reduces Streptococcus pyogenes virulence by modulating the IL-17, IL-23 and Toll-like receptor 2/4 expressions in human epithelial cells. Int Immunopharmacol2013; 17(2): 453–61.
60.    S. Ahrné, S. Nobaek, B. Jeppsson, I. Adlerberth, A. E. Wold, and G. Molin, “The normal Lactobacillus flora of healthy human rectal and oral mucosa, ” Journal of Applied Microbiology, vol. 85, no. 1, pp. 88–94, 1998.
61.    S. P. Borriello, W. P. Hammes, W. Holzapfel et al., “Safety of probiotics that contain lactobacilli or bifidobacteria, ” Clinical Infectious Diseases, vol. 36, no. 6, pp. 775–780, 2003.
62.    Sharma C, Singh BP, Thakur N, et al. Antibacterial effects of Lactobacillus isolates of curd and human milk origin against foodborne and human pathogens. 3 Biotech 7 (2017): 31.
63.    Shokryazdan P, Sieo CC, Kalavathy R, Let al. Probiotic potential of Lactobacillus strains with antimicrobial activity against some human pathogenic strains. BioMed research international (2014).
64.    Soundharrajan Ilavenil, Hyung Soo Park, Mayakrishnan Vijayakumar, MariadhasValan Arasu, Da Hye Kim, Sivanesan Ravikumar, and Ki Choon Choi. Probiotic Potential of Lactobacillus Strains with Antifungal Activity Isolated from Animal Manure.The Scientific World Journal, Volume 2015.
65.    Tan P, Eor J, Chun T, et al. Immune Modulation by Probiotics. In: Liong MT. (eds) Beneficial Microorganisms in Medical and Health Applications. Microbiology Monographs 28 (2015): 110-130.
66.    Terahara M, Kurama S, Takemoto N (2001). Prevention by lactic acid bacteria of the oxidation of human LDL. Biosci Biotechnol Biochem, 65(8): 1864-68.
67.    Todorov SD, Furtado DN, Saad SMY, et al. (2011) Bacteriocin production and resistance to drugs are advantageous features for Lactobacillus acidophilus La-14, a potential probiotic strain. New Microbiologica 34: 357–370
68.    Tsai CC, Lin PP, Hsieh YM. Three Lactobacillus strains from healthy infant stool inhibit enterotoxigenic Escherichia coli grown in vitro. Anaerobe 14 (2008): 61-67.
69.    U. Schillinger and J. V. Villarreal, “Inhibition of Penicillium nordicum in MRS medium by lactic acid bacteria isolated from foods, ” Food Control, vol. 21, no. 2, pp. 107–111, 2010.
70.    Usman and Hosono, 1999Usman and Hosono, A. Bile tolerance, taurocholate deconjugation, and binding of cholesterol by Lactobacillus gasseri strains. J. Dairy Sci. 1999; 82: 243–248 (10068945)
71.    Vizoso Pinto et al., 2006Vizoso Pinto, M.G., Franz, C.M., Schillinger, U., and Holzapfel, W.H. Lactobacillus spp. within vitro probiotic properties from human faeces and traditional fermented products. Int. J. Food Microbiol. 2006; 109: 205–214 (16503361.
72.    Vos P, Garrity G, Jones D, et al. Bergey's manual of systematic bacteriology: Volume 3: The Firmicutes. Springer Science and Business Media (2011).
73.    W. H. Holzapfel, P. Haberer, J. Snel, U. Schillinger, and J. H. Huis in't Veld, “Overview of gut flora and probiotics, ” International Journal of Food Microbiology, vol. 41, no. 2, pp. 85–101, 1998.
74.    Wang X, Hou JM, Fu LL, Yang SD, Zhang X, Li D, Zhang ZN. In vitro screening of lactic acid bacteria producing β-galactosidase and their probiotic characteristics. China Dairy Ind 2012; 40: 20-23.
75.    Wang ZL, Fu LY, Xiong ZA, et al. (2016) Diagnosis and microecological characteristics of aerobic vaginitis in outpatients based on preformed enzymes. Taiwan Journal of Obstetrics and Gynecology 55: 40–44
76.    Wayne PA. CLSI. Performance standards for antimicrobial susceptibility testing. 26th ed. CLSI supplement M100S.
77.    WHO F. Report on Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria (2001).
78.    Wu R, Wang L, Wang J, Li H, Menghe B, Wu J, Guo M, Zhang H. Isolation and preliminary probiotic selection of lactobacilli from koumiss in Inner Mongolia. J Basic Microbiol 2009; 49: 318-326. 8
79.    Yu Qian, XingyaoLong, Yanni Pan, Guijie Li, and Xin Zhao. Isolation and identification of lactic acid bacteria (Lactobacillus plantarum YS2) from yak yogurt and its probiotic properties. Biomedical Research (2018) Volume 29, Issue 4. DOI: 10.4066/biomedicalresearch.29-17-3418
80.    Yueyue M, Li Z, Guicheng H. Effects of ten strains of Lactobacillus on the activity of immune cells in vitro. Sci Technol Food Ind (in Chinese, 2018; 39(01): 312–317).
81.    Yueyue Meng, Bailiang Li, Da Jin, Meng Zhan, Jingjing Lu, and GuichengHuo, Immunomodulatory activity of Lactobacillus plantarum KLDS1.0318 in cyclophosphamide-treated mice.

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